July 20, 2010
Lab 2
Histology:
-looked at slides of:
Abalone (healthy)
-identified: mature oocytes, digestive gland tubules, gill tissue, muscle, intestine and stomach
Oyster (healthy)
-identified: digestive gland tubules, gill tissue, muscle, intestine and oocytes
Flat Oyster (Bonamia)
-identified: Bonamia parasite in the gonads around/ between the oocytes and spermatids (hermaphrodite)
Oyster (Ancistroma)
-identified: Ancistroma parasite in the anastomosing epithelium of the digestive gland, digestive gland tubules and Brown cells with little blobs inside indicating that this was the Pacific oyster
Littorina dissection:
- dissected two species of Littorina: L. scutulata (checkered) and L. sikana (striped) collected form Cattle Point
methods:
1) collected organism, cracked shell with a vice, removed shell
2) cut off head, digestive gland and operculum
3) placed remaining body tissue in a RNase free labeled tube and put in the -20C freezer in the dry ice box for RNA extraction
4) cut off a small piece of foot from the operculum for DNA analysis, placed in labeled tube and put in the -20C freezer
5) observed digestive gland under the dissecting scope to check for parasites
- I dissected and processed 3 L.sikana: organisms appeared healthy with no obvious signs of infection
- I dissected and processed 3 L. scutulata: organisms appeared healthy with no obvious signs of infection
- other lab mates found trematode infections of varying degrees upon observation of the digestive glands in both species
- the trematodes found differed and some interesting parasitic features included: suckers, eye spots, tail spikes